秸秆是广泛存在的可再生生物质资源之一,但由于木质素复合体的难降解性,当前湿式厌氧发酵对秸秆利用程度普遍较低。 本研究通过将一种具有高效降解木质素能力的白腐菌———黄孢原毛平革菌与厌氧污泥在微氧条件下共培养,并通过秸秆的湿式发酵,探究共培养对秸秆悬液产甲烷的影响。 结果表明,在 0.6 mg / L 溶解氧条件下,与非共培养组相比,共培养组的底物甲烷产率提高了 45%(达到 75.76 mL/ g VS),TCOD 去除率提高了 37%。 电化学分析结果显示,共培养能有效提高污泥电容(提高了 182.9%)。 黄孢原毛平革菌的引入使秸秆更易被利用,并且秸秆代谢产生的电活性物质加强了腐殖化过程及污泥胞外电子传递,从而增加了甲烷的产量。 本研究实现了对未经预处理的秸秆高效厌氧发酵,为秸秆资源化提供借鉴。

Straw is one of the widely available renewable biomass resources. However, due to the diffi⁃cult degradability of the lignin structure, wet anaerobic fermentation is generally not highly utilized forstraw at present. In this study, Phanerochaete chrysosporium, a white-rot fungus, was co-cultivatedwith anaerobic sludge under microaerobic conditions to investigate its effect on methane production fromstraw in wet anaerobic fermentation. The results showed that the substrate methane yield (75.76 mL/ g VS)and TCOD removal in the co-culture group increased by 45% and 37%, respectively, compared to thenon-co-culture group at 0.6 mg/ L dissolved oxygen. Electrochemical analysis revealed that co-cultureeffectively increased the sludge capacitance by 182.9%. The introduction of Phanerochaete chrysosporiummade the straw easier to digest and produced electroactive substances, which intensified the humifica⁃tion process and extracellular electron transfer, and consequently increased methane production. Effec⁃tive utilization of straw without pretreatment was achieved in this study, providing a reference for furtherstraw resource recovery.