煤炭开采造成矿区土地生产力降低，生态脆弱问题加剧，微生物联合植物复垦技术是矿区生态修复常用手段，合适的菌剂接种方式对加快矿区生态修复具有重要意义。深色有隔内生真菌(Dark Septate Endophytes，DSE)是一类普遍存在于植物根系的内生真菌，其代谢物具有植物生长刺激素的功能。以紫花苜蓿为研究对象，在浸根方式和模拟土培方式2种接种方式下，设置DSE菌丝（HD）、代谢物（MD）、菌丝及其代谢物（HD+MD）及空白对照（CK）4个处理，探究DSE及其代谢物对紫花苜蓿的促生作用机制。结果表明：浸根方式下菌丝及其代谢物联合处理的紫花苜蓿长势最佳，总生物量、总叶绿素、根体积、全氮含量分别比对照组增加了83.78%、35.54%、132.12%、82.16%，DSE在紫花苜蓿根系定殖率可达68.89%，且以菌丝结构定殖为主。模拟土培方式下DSE代谢物对紫花苜蓿的促生作用显著，定殖以微菌核结构为主。相关性分析表明，与接种方式相比较，DSE接种处理与菌丝定殖率、微菌核定殖率、总定殖率、根长、根表面积、根投影面积呈显著正相关(P<0.05)，与地上部鲜重、根鲜重、总鲜重、根体积、全氮、全钾呈极显著正相关(P<0.01)。DSE及代谢物通过定殖率和光合作用调控紫花苜蓿的生长，为矿区生态修复绿色菌肥的的开发利用提供了理论依据。

Coal mining has caused the reduction of land productivity in mining areas and exacerbated the problem of ecological fragility, while microbial combined plant reclamation technology is a commonly used means of ecological restoration in mining areas, and the appropriate inoculation method of microbial agents is of great significance for accelerating the ecological restoration in mining areas. Dark Septate Sndophytes (DSE) are a group of endophytic fungi ubiquitously existing in plant root systems, whose metabolites could function as plant growth stimulants. Taking Medicago sativa L. as the research object, four treatments were set up, including DSE hypha (HD), metabolite (MD), hypha and its metabolite (HD+MD) and blank control (CK), to explore the mechanism of DSE and its metabolite promoting effect on Medicago sativa L., under two inoculation methods of root dipping and simulated soil cultivation. The results showed that Medicago sativa L. co-treated with hypha and its metabolites under root dipping method had the best growth potential. Compared to the control group, the total biomass, total chlorophyll, root volume and total nitrogen content increased by 83.78%, 35.54%, 132.12% and 82.16%, respectively. The colonization rate of DSE in the root system of Medicago sativa L. could achieve 68.89%, dominated by the colonization in the hypha mode. Under simulated soil cultivation, DSE metabolites performed a significant growth-promoting effect on Medicago sativa L., and the colonization was dominated by the microsclerotia mode. The correlation analysis showed that compared with the inoculation method, the DSE inoculation treatment was significantly positively correlated with hypha colonization rate, microsclerotia colonization rate, total colonization rate, root length, root surface area, root projected area (P<0.05), and highly significantly positively correlated with aboveground fresh weight, root fresh weight, total fresh weight, root volume, total nitrogen and total potassium (P<0.01). DSE and its metabolites could regulate the growth of Medicago sativa L. through colonization rate and photosynthesis, providing a theoretical basis for the development and utilization of green microbial fertilizer for ecological restoration in mining areas.