Shandong Benyuan Environmental Technology Co., Ltd.
School of Chemistry andEnvironmental Engineering, China University of Mining and Technology (Beijing)

随着我国逐步成为最大能源消费国与碳排放国,CO2的高效资源化利用成为实现“双碳”战略的重要手段。微生物催化还原CO2备受关注,可低成本同步实现绿氢储存和CO2转化利用。围绕CO2干式生物甲烷化方法的建立,从转化速率的关键影响因素和微生物群落结构两方面开展实验研究。研究结果表明,在无氧干式反应器中,以载碳海绵为填料,温度设置为35℃,最佳H2/CO2进料比为4∶1,最佳营养液浸泡周期为6d。通过高通量测序技术对浸泡周期结束的微生物进行测序分析,发现在干式反应器中,厌氧耗氢产甲烷菌是主导,属水平上以Methanobacterium和Methanosaeta为主,细菌群落中Acetobacterium和Mesotoga丰富度占比较高。对CO2干式生物甲烷化方法的转化速率的关键影响因素和微生物群落结构进行研究,为微生物产甲烷提供了理论基础和技术支撑。

As China has gradually become the largest energy consumer and carbon emitter, the efficientutilization of CO2 has become an important means in realizing the " dual carbon" strategy. Microbialcatalytic reduction of CO2 offers a low-cost solution for simultaneous green hydrogen storage and CO2conversion and utilization. This paper focuses on the establishment of CO2 dry biomethanization method,and conducts experiments to identify the key factors affecting the conversion rate and microbial commu-nity structure. The results showed that maintaining a temperature of 35 ℃, employing an optimal H2 /CO2 feed ratio of 4 ∶ 1, and implementing a 6-day soaking period for the nutrient solution in an anaero-bic dry reactor filled with carbon sponge are critical for enhancing the conversion rate. Additionally,microbes at the end of the soaking cycle were sequenced and analyzed by high-throughput sequencingtechnology. The dry reactor exhibited dominance by anaerobic hydrogen-consuming methanogens, par-ticularly at the genus level with Metanobacterium and Metaosaeta being prevalent. Moreover, the bacte-rial community displayed relatively high levels of Aconobacterium and Mesotoga. This study aims to in-vestigate the key factors affecting the conversion rate of CO2 dry anaerobic biomethanation and to ana-lyze the structure of the microbial community, in order to provide a theoretical foundation and technicalsupport for the process of microbial methane production.

国家自然科学基金资助项目(51978658)

谢昕,王春辉,于荣珍,等.CO2干式生物甲烷化试验研究[J].能源环境保护,2024,38(3):109-116.

XIE Xin, WANG Chunhui, YU Rongzhen, et al. Experimental study on CO2 dry biomethanation[J]. Energy En-vironmental Protection, 2024, 38(3): 109-116.