1. State Key Laboratory of Coal Resources and Safe Mining,China University of Mining and Technology ( Beijing),Beijing　 100083,China;2. Kazakh Na-tional Research Technical University named after K. I. Satpayev,Kazakh　 040900,Kazakhstan

三维激光扫描技术建立的植物三维立体模型,可精确、全面、原位地反映植物形态特征,对于揭示接菌植物的微观形态特性具有比传统测量方法更明显的优势。以玉米为供试材料,在低磷条件下(20 mg/kg),设置接种菌根与对照两个处理,研究三维激光扫描技术对接种丛枝菌根真菌的植株形态特征在不同生长期的动态变化规律,分析比较不同处理的植株侵染率、菌丝密度、土壤酸性磷酸酶活性和土壤有效磷等理化指标及玉米形态特征的变化。研究结果表明,在低磷条件下丛枝菌根在30 d时侵染率已经达到82%,菌丝密度增加,酸性磷酸酶活性增强。在生长到30 d的时候,接菌组的株高、冠幅分别比对照组提高52%,47%,生长到60 d时接菌组茎杆横截面积为对照组的124%,玉米活叶面积、叶宽、叶片垂高等都得到提高,并且随着生长期延长接菌和对照处理的差异增大。以曲率半径表示叶片弯曲程度,在生长到60 d时接菌组曲率半径比对照组增加46.5%,接种丛枝菌根真菌促使叶片更加坚挺。

The 3D model of plant based on 3D laser scanning technology can reflect plant morphological characteristics accurately and comprehensively without artificial disturbance. It has obvious advantages over the traditional measure- ment method. Maize grown under low phosphorus conditions (20 mg / kg). The inoculation ( Glomus intraradices) treatment and blank control treatment were established. A 3D laser scanning technology was used for dynamic monito- ring on the maize morphology in different growth times. Considering the infection rate,hyphal density,acid phosphatase activity,available phosphorus and other physicochemical indexes,the change law of the effect of arbuscular mycorrhizal fungi on the growth of maize was summarized. The results show that under low phosphorus conditions,the infection rate reaches 82% in 30 days,and the hyphal density and the acid phosphatase activity also increase. When the maize grows for 30 days,the plant height and crown width in the inoculation group increase by 52% and 47% respectively com- pared with that in the control group. While in 60 days,the cross-sectional area of stem in the inoculation group is 124% of that in the control group. Moreover,the leaf area,leaf width and the vertical height of the blade and so on all increase,and the difference between inoculation treatment and CK treatment is increased with time increase. Radius of curvature was used to express the leaf bending degree. When the corn grows for 60 days,the curvature radius in the in- oculation group increases by 46． 5% compared with that in the control group,which indicates that the arbuscular my- corrhizal fungi can strengthen the leaf.

国家自然科学基金资助项目(51574253)；煤炭资源与安全开采国家重点实验室重点资助项目(SKLCRSM16KFA01)；大学生创新基金资助项目(C201502003)；

Bi Yinli,Yu Haiyang,Yryszhan Zhakypbek,et al. Morphological characteristics of mycorrhizal plant based on 3D laser scanning technology [J]. Journal of China Coal Society,2016,41(8):2071-2078.