厌氧发酵产甲烷过程及结果与微量元素及络合物的存在密切相关，为了研究Fe2+、Ni2+及络合物对煤制生物甲烷生成的影响以及产气过程中参数的变化,采用对比和放大试验的方法，以义马煤矿低煤阶煤为研究对象，矿井水作为菌种源，设置4种不同的方案，分别记录其产气数据和COD，并利用改进的Gompertz模型对各试验方案的产甲烷及降解过程进行模拟。试验结果表明：对比试验中加有15 mg/L的Fe2+和0.005 mg/L的Ni2+对生物产甲烷具有促进作用，有利于提高煤生物制甲烷的产量以及对有机质的降解，在上述试验基础上再加入5 mg/L的EDTA可以更好地促进产气，并使底物降解率更高，而放大试验的产气效果和底物降解率均要优于小样试验；改进的Gompertz模型对各试验方案的产甲烷及降解过程模拟结果得出，在加有Fe2+、Ni2+和EDTA的小样试验中，其产甲烷潜势（24.344 59 mL/g）、最大比产甲烷率（0.765 24 mL/(g·d)）、底物降解率（4 535 mg/L）及最大底物降解速率（197.8 mg/(L·d)）等参数都是最优的，且放大试验的以上参数均要优于小样试验。对各试验方案建立了相应的动力学方程，用数理统计方法对其进行检验，得出所建立的回归方程是可靠且高度显著的。该试验及其动力学研究有助于了解微量元素及络合物对煤制生物甲烷的影响，并有助于对产气过程中的各参数变化有进一步的认识，为煤制生物甲烷工程化提供一定的试验基础。

The process and results of anaerobic fermentation of methane production are closely related to the existence of trace elements and complexes, in order to study the influence of Fe2+, Ni2+ and complexes on the production of coal-based biomethane and the changes of parameters in the process of gas production, using the method of comparison amplification experiments, the low-rank coal in Yima coal mine was taken as the research object, and the mine drainage was used as the strain source. Four different schemes were set up to record their gas production data and COD value respectively, and the improved Gompertz model was used to simulate the methane production and degradation process of each experimental scheme.The test results show that the addition of 15 mg/L of Fe2+ and 0.005 mg/L of Ni2+ in the comparative testhas a promoting effecton biomethane production, which is beneficial to increaseoutput of coal bio-methane production as well as the degradation of organic matter.On the basis of the above experiment,the addition of 5 mg/L of EDTA can better promote the gas production and make the substrate degradation rate higher, the gas production effect and substrate degradation rate of the scale-up test are better than those of the sample test; the improved Gompertz model for the experimental scheme of methanogenesis and degradation process of the simulation results show that Fe2+ and Ni2+ and EDTA are with small sample test, its methane production potential (24.344 59 mL/g), and the maximum methane production rate (0.765 24 mL/(g·d)), the substrate degradation rate (4 535 mg/L) and the maximum substrate degradation rate (197.8 mg/(L·d))and other parameters are optimal, and the above parameters of the scale-up test should be excellent for sample test. The corresponding kinetic equations of each experimental scheme were established and tested by mathematical statistics method. The results show that the established regression equations were reliable and highly significant. The experiment and its kinetics study are helpful to understand the influence of trace elements and complexes on coal-to-biomethane, and to have a further understanding of the changes of various parameters in the process of gas production, providing a certain experimental basis for the engineering of coal-to-biomethane. 

0 引言

1 试验材料与方法

   1.1 试验材料

   1.2 试验步骤

   1.3 试验设计

2 试验结果

   2.1 产甲烷试验结果

   2.2 COD降解效果对比

3 动力学过程研究

   3.1 动力学曲线模拟

   3.2 动力学模型的建立

   3.3 模型检验

4 分析与讨论

5 结论