Engineering Research Center of Ministry of Education for Mine Ecological Restoration
Carbon Neutrality Institute, ChinaUniversity of Mining and Technology
CUMT-UCASAL Joint Research Center for Biomining and Soil Ecological Restoration,China University of Mining and Technology

Vegetation restoration is an important indicator of ecosystem health in a mining area. Understanding the impactof vegetation restoration on the characteristics and assembly process of soil microbial community is very important to explorethe resilience and self-sustaining mechanism of the restored ecosystem in a mining area. Therefore, this study uses MiSeq high-throughput sequencing and zero model to detect the composition of soil microbial communities, the characteristicsof molecular ecological network, the key flora and its assembly mechanism in the shrubs (BL), coniferous forests(CF), broad-leaved forests (BF), mixed forests (MF), that have been reclaimed for 18 years in Antaibao open-pit miningdump, and the control plots (CK, undisturbed surrounding poplar forests that have continued to grow for more than 30years) in Pingshuo, Loess Plateau. The results show that: ① The effects of different vegetation restoration types on thebacterial community α-diversity are significant (P < 0.05). Compared with CK, the Sobs and Shannon index of MF and CFhave increased by 35.29%, 3.50% and 25.18%, 1.05%, respectively, whereas there is no significant difference in the α-diversityof fungal community among different vegetation restoration types. ② Actinobacteria, Chloroflexi, Proteobacteriaand Acidobacteria dominate in the bacterial community, and the first two dominant phylum are significantly higher thanCK, while the latter two are opposite (P < 0.05). Ascomycota and Basidiomycota are the dominant fungi in the fungal community.The former is significantly higher than CK, while the latter is opposite (P < 0.05). ③ The stochastic process dominatesthe construction process of the soil bacterial community. In addition to the MF soil fungal community dominated bydeterministic process, other fungal communities are also dominated by a stochastic process. However, no matter whichtype of vegetation is restored, the dominant role of the randomness process on the assembly of the bacterial community ismuch higher than that of the fungal community. Moreover, Proteobacteria and Acidobacteria are key taxa of the bacterialnetwork, while Mortierellales, Thelebolales, Chaetothyriales, and Hypocreales are the key taxa of the fungal network. Vegetationrestoration affects microbial community diversity, BL, CF, and MF increase the stability of the bacterial network,and MF makes fungal network more complex. The soil bacterial assembly process is dominated by stochastic processes,except for MF, and the fungal assembly process is also dominated by stochastic processes.

国家自然科学基金资助项目(51974313，41907405)

杨永均，赵姣，马静，等. 露采矿山复绿对土壤微生物群落及其组装过程的影响[J]. 煤炭学报，2023，48(4)：1661−1672

YANG Yongjun，ZHAO Jiao，MA Jing，et al. Impacts of regreening on soil microbial community and its assembly process in open-pit mining area of the Loess Plateau[J]. Journal of China Coal Society，2023，48(4)：1661−1672