Institute of Resources & Environment,Henan Polytechnic University
Provincial Collaborative Innovation Center of Coal Produc⁃tion Safety and Clean and Efficient Utilization
Research Institute of Unconventional Natural Gas,Henan Polytechnic University
School of Energy Science and Engineering,Henan Polytechnic University
China Petroleum Explorationand Development Institute

煤制生物气过程主要依靠细菌和古细菌的共同作用完成,产气是目的,煤的底物利用率是关键。而水解是整个发酵过程的限速步骤,关系到煤的水解率及降解率,水解细菌作为煤制甲烷厌氧发酵过程的重要功能菌群,研究优势水解菌种的特征降解功能具有特殊重要意义。以水解过程中的细菌为研究对象,分析各单一菌种及复合菌剂对煤的降解特征,并探讨菌种间的共轭关系,结果如下:不动杆菌和假单胞菌联合降解后在进行产气的样品,其总产气量最高为19.86mL/g,假单胞菌处理后的样品其总产甲烷量最高,总产甲烷量为10.78mL/g;不动杆菌和假单胞菌联合降解,其降解特征与不动杆菌降解功能相似;不动杆菌和多粘类芽孢杆菌联合降解,失去对羧酸中OH键和单邻芳香C—H键降解能力;假单胞菌和多粘类芽孢杆菌联合降解,减弱了对羧酸中OH键、单邻芳香C—H键的降解能力;假单胞菌降解后的煤样其比表面积和总孔容降低程度最大,分别降低了16.478m2/g和0.284cm3/g。经不同菌剂降解后介孔和大孔平均孔径普遍增大,而微孔最可几孔径普遍减小。本研究为提高煤的水解率和最终的产甲烷能力提供支撑。

The process of coal⁃to⁃biogas mainly relies on the joint action of bacteria and archaea,gas production is thepurpose,and the substrate utilization of coal is the key. The hydrolysis is the rate⁃limiting step of the whole fermenta⁃tion process,which is related to the hydrolysis rate and degradation rate of coal. Hydrolysis bacteria,as an importantfunctional group of coal⁃to⁃methane anaerobic fermentation process,are of special importance for studying the charac⁃teristic degradation function of the dominant hydrolysis strains. In this study,the degradation characteristics of coalwere analyzed by each single strain and compound bacterial agent with the bacteria in the hydrolysis process,and the conjugation relationship between the strains was explored. The results were as follows:the sample after com⁃bined degradation of Bacillus immobilis and Pseudomonas aeruginosa in gas production showed the highest total gasproduction,with the total gas production of 19.86 mL/ g. The sample after Pseudomonas treatment showed the high⁃est total methane production, with the total methane production of 10. 78 mL/ g. The degradation characteristicsof the combined degradation of Bacillus immobilis and Pseudomonas were similar to those of Bacillus immobilis.The combined degradation of Bacillus immobilis and Bacillus polymyxa lost the degradation ability of OH bond andsingle⁃neighboring aromatic C—H bond in the carboxylic acid. The combined degradation of Pseudomonas andBacillus polymyxa weakened the degradation ability of OH bond and single⁃neighboring aromatic C—H bondin the carboxylic acid. The specific surface area and the total pore volume of the coal samples degraded by Pseudo⁃monas decreased the most,which decreased by 16.478 m2 / g and 0.284 m3 / g,respectively. The average pore sizeof mesopores and macropores generally increased after being degraded by different bacterial agents,while the most poresize of micropores generally decreased. This study provides some references for the improvement of the hydrolysis rateand the final methane production capacity of coal.

国家自然科学基金资助项目(42172199);河南省优秀青年培育基金资助项目(202300410168);河南省青年骨干基金资助项目(2018GGJS059)

夏大平,赵彦翔,苏现波,等. 不同水解菌及复合菌剂对长焰煤作用特征[J].煤炭学报,2023,48(S1):185-193.

XIA Daping,ZHAO Yanxiang,SU Xianbo,et al. Characterization of the action of different hydrolytic bacteriaand compound bacterial agents on long⁃flame coal[J]. Journal of China Coal Society,2023,48(S1):185-193