School of Chemical Engineering, University of Science and Technology Liaoning

焦化废水中的吡啶具有较强的毒性、致畸性和致癌性且难以被有效降解,因而采用菌株Achromobactersp.与葡萄糖共代谢作用以探究高效降解废水中的吡啶具有重要意义。结合菌种的鉴定及GC-MS的测定,剖析其代谢产物与吡啶的代谢途径,并利用Haldane模型对Achromobact⁃ersp.生长动力学进行分析,研究生物强化处理焦化废水以及Achromobactersp.对吡啶的降解特性,探究菌株Achromobactersp.对工业废水中难降解物质吡啶的降解,得知最适该菌降解吡啶的条件为初始质量浓度400mg/L和接种量10%(体积比)。在此基础上,添加150mg/L葡萄糖为共代谢底物,对吡啶降解率在32h可达98.06%。结合GC-MS分析,菌株通过吡啶环上N和C2之间断裂生成中间产物NH+4和戊二醛来降解吡啶。吡啶初始浓度为100~700mg/L,菌株生长动力学符合Haldane模型,最大比生长速率(μmax)为0.61h-1,底物抑制系数(Ki)为734.01mg/L。在葡萄糖共代谢作用下,Achromobactersp.与活性污泥耦合使焦化废水中的吡啶完全降解时间由40h减少至24h,TOC降解率由84.31%提高至91.44%。菌株Achromobactersp.可用于含有吡啶废水的高效处理,为其生物修复提供1种新途径。

The pyridine in coking wastewater has strong toxicity, teratogenicity, and carcinogenicity, and it is diffi⁃cult to be effectively degraded. Therefore, it is of great significance to explore the efficient pyridine degradation inwastewater by Achromobacter sp. with glucose co⁃metabolism. Combining with the bacteria identification and Haldanemodel, the pyridine metabolites and its metabolic pathway were analyzed. The growth kinetics of Achromobacter sp.was analyzed using the Haldane model. The bio⁃augmentation treatment of coking wastewater and the degradationcharacteristics of Achromobacter sp. for pyridine were studied. Research on how the Achromobacter sp. degradates theof pyridine (a difficult to degrade substance in industrial wastewater) was done, we can see that he optimal condi⁃tions for pyridine degradation by this strain are an initial concentration of 400 mg / L and an inoculation volume of 10%(volume ratio). On this basis, 150 mg / L glucose as the co⁃metabolism substrate is added into the solution, the pyri⁃dine degradation efficiency can reach 98. 06% in 32 h. GC⁃MS analysis shows that the pyridine metabolic pathway isthrough breaking the chemical bond between N and C2 on the pyridine ring to produce intermediate NH+4 and glutaral⁃dehyde. The bacteria growth kinetics conform to Haldane model in initial pyridine concentration of 100 mg / L to700 mg / L. The maximum specific growth rate ( μmax ) is 0. 61 h-1 and substrate inhibition coefficient ( Ki ) is734. 01 mg / L. Based on glucose co⁃metabolism, the coupling of Achromobacter sp. and activated sludge shortens thepyridine removal time in coking wastewater from 40 h to 24 h, and the TOC degradation efficiency increases from84. 31% to 91. 44%. The strain Achromobacter sp. can effectively be used for pyridine⁃containing wastewater treat⁃ment, which provides a new way for its bioremediation.

辽宁科技大学研究生科技创新资助项目(LKDYC202119)

冯晓娇, 邓铭, 迟海军, 等. 葡萄糖共代谢作用下 Achromobacter sp. 对焦化废水中吡啶的生物降解特性[J]. 煤质技术, 2023, 38 (3): 28-36.

FENG Xiaojiao, DENG Ming, CHI Haijun, et al. Achromobacter sp. biodegradation characteristics for pyridine incoking wastewater based on glucose co⁃metabolism [J]. Coal Quality Technology, 2023, 38 (3): 28-36.